The results indicated that the expression of ANKLE1 mRNA correlated with DNA methylation, but not with CNV (r = 0.08451, p = 0.0611) and gene mutation (r = − 0.07674, p = 0.0884) in PRAD (Fig. 8A), and the expression of EMD mRNA was correlated with DNA methylation and positively correlated with CNV (r = 0.1583, p < 0.001), but not with a gene mutation in PRAD (Fig. 8B). The gene discussed is ANKLE1; the disease is prostate adenocarcinoma.