The results showed that ANKLE1 expression was significantly positively correlated with NK CD56bright cells (r = 0.095, p = 0.034) and CD8 T cells (r = 0.187, p < 0.001) in PRAD (Fig. 5A), and ANKLE1 expression was significantly negatively correlated with macrophages (r = − 0.165, p < 0.001), neurophils (r = − 0.141, p = 0.002), mast cells (r = − 0.105, p = 0.02), and Th17 cells (r = − 0.171, p < 0.001) in PRAD (Fig. 5A). The gene discussed is ANKLE1; the disease is prostate adenocarcinoma.