In addition, we found that the levels of ANKLE1, EMD, and LEMD2 were significantly correlated with various subtypes of T cell marker levels, including CD8+ T cell markers (CD8A, CD8B), general T cell markers (CD3D, CD3E, CD2), exhausted T cell marker (GZMB, LAG-3, PD-1), Th2 markers (GATA3), Th17 markers (STAT3), Treg markers (FOXP3, CCR8, TGF-b), Tfh marker (BCL6), neutrophils markers (ITGAM, CCR7), DC markers (CD1C, ITGAX), B cells markers (CD79A, CD19), and macrophages in PRAD (Additional file 1: Table S3) by TIMER web server. The gene discussed is CD3D; the disease is prostate adenocarcinoma.