TRPV1 expression is modulated by inflammatory mediators, given that the TG is outside the blood brain barrier, this suggests that the circulating inflammatory milieu in obesity could be causative in the upregulation of TRPV1, given that our qPCR data suggests no increases in pro-inflammatory gene expression in the TG22,43. The gene discussed is TRPV1; the disease is obesity due to melanocortin 4 receptor deficiency.