KDM5B and melanoma: To create an experimental model that allows the exogenous induction of KDM5B protein expression, we cloned a Tet-On 3G-system, in which KDM5B expression is driven by a doxycycline-inducible PTRE3G promoter and established stable melanoma cell clones (WM3734Tet3G-KDM5B and WM3734Tet3G-EGFP control, Supplementary Fig. 2a–c).