CASP3 and melanoma: Standard proliferation (MTT) and apoptosis (caspase 3 plus annexin V measurement or 7AAD flow cytometry) assays indicated a rather cytostatic than apoptotic or toxic effect in short-term Cpd1-treated melanoma cell lines or WM3734Tet3G-KDM5B cells (Fig. 2a, left panel and Supplementary Fig. 5a, b).