To obtain a second, independent method for the modulation of KDM5B expression, which additionally allows simple application to several melanoma cell lines or in vivo, we developed a cell-based compound screening assay applying our previously published KDM5B-promoter-EGFP-reporter construct stably expressed in WM3734 melanoma cells (WM3734KDM5Bprom-EGFP cells15, Supplementary Fig. 3a). The gene discussed is KDM5B; the disease is melanoma.