Notably, although we provide evidence to demonstrated that METTL14-mediated m6A modification repressed the MACF1/β-catenin pathway in CCA, while METTL14R298H mutation disrupted this mechanism, m6A-related key catalytic protein would be more complicated on the WNT pathway and the underlying mechanism is not fully characterized. Here, METTL14 is linked to cholangiocarcinoma.