In this study, we show that a 48-hour RA pulse in combination with SHH pathway activation promote a rapid specification of LMX1A+/FOXA2+/OTX2+ vMB progenitors that differentiate into functional mDA neurons at high yield in vitro, and which engraft and restore motor deficits after transplantation into a rat model of PD. This evidence concerns the gene OTX2 and Parkinson disease.