However, 4βOHC concentrations were lower in patients with obesity expressing CYP3A4 *1/*22 compared with CYP3A4 *1/*1 (p = 0.024), whereas in the normal to overweight individuals, the difference did not reach statistical significance (p = 0.12) (Supplementary Fig. S6c and d). The gene discussed is CYP3A4; the disease is obesity due to melanocortin 4 receptor deficiency.