PPIG and chronic cystitis: These findings are in accordance with those reporting elevated AEA levels and bladder inflammation in rats, but not in mouse models for cystitis.14,21,22 However, in contrast to others, they showed no changes in 2-AG levels in a CYP-induced mouse model for cystitis.22 These discrepancies may be related to differences in the model established, the number of mice used in each experiment, the eCB extraction method, and the normalization method used for calculating eCB levels.