Inspired by elegant work that uncovered molecular determinants of TDP-43 LLPS and fibrillization in vitro (Conicella et al., 2016; Li et al., 2018b; Schmidt et al., 2019; Pantoja-Uceda et al., 2021), in the present study we sought to determine whether 1) different ALS-linked TDP-43 mutations or 2) disruption of specific aromatic and charged residues in the LCD have distinct effects on the formation of TDP-43 positive RNP condensates and their functional impact on TDP-43 RNP transport in neurons. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.