Finally, combining the expression of 6-LRLs and regression coefficients (Figure 3D), the risk score of ESCC patients is calculated as follows: Risk score = (0.3551 * TMEM161B-AS1) + (−0.3214 *C8orf49) + (1.0463 * LINC01588) + (−0.1548 * LINC02489) + (0.4666 *C9orf147) + (0.2409 * AC126773.3). The gene discussed is HSDL2-AS1; the disease is esophageal squamous cell carcinoma.