In consistence with the RNA-seq data, qRT-PCR analysis of clinical tissue samples also showed that the expression of C8orf49, LINC01588, LINC02489, C9orf147, and AC126773.3 was increased and the expression of TMEM161B-AS1 decreased in ESCC tissues compared with adjacent normal esophageal tissues (Figure 11B). This evidence concerns the gene HSDL2-AS1 and esophageal squamous cell carcinoma.