APOB and hepatocellular carcinoma: So that gene expression can match the in vivo level, Takahashi et al. (2015) (93) demonstrated that when hepatoma cell lines like HepG2 and Hepa RG were cultured using the hanging drop method, the expression of genes related to glucose, lipid, and drug metabolism was remarkably increased, the secretion of albumin and Apo B (Apolipoprotein B) was enhanced, and the mRNA levels of CYP (cytochrome P450) enzymes, on exposure to specific inducers, increased.