AKT1 and neoplasm: In LIHC with high CHRNE expression, 150 genes were unregulated and 16 genes were downregulated (FC>2, P<0.05) (Figures 6A, B), while a low expression of CHRNE had an upregulation of genes associated with physiological functions of the liver (e.g., bile secretion, cholesterol metabolism, drug metabolism) and activation processes, such as the regulation of coagulation; downregulation of tumor-promoting pathways, including the PI3K-Akt signaling pathway; and suppression of processes like transition metal ion homeostasis (Figures 6C, D).