We found that the phosphorylation of AKT1/2 (S473) and GSK3α/β (S21/S9) was upregulated, along with the overexpression of FAM222A-AS1 in CRC cells (Figure S4A), which indicates that FAM222A-AS1 may regulate CRC cell proliferation and invasion via downstream AKT- and GSK3α/β-associated pathways. This evidence concerns the gene AKT1 and colorectal carcinoma.