Our human phosphorylation protein screening assay indicated that FAM222A-AS1 was significantly associated with the variations in AKT1/2 (S473) and GSK3α/β phosphorylation, implying that FAM222A-AS1 may regulate the progression of CRC via AKT1/2 and GSK3α/β-associated signaling pathways, which needs to be verified in a future study. The gene discussed is AKT1; the disease is colorectal carcinoma.