Simultaneously, based on the COSMIC mutation database, no hotspot region was observed for FAT1 mutations in HNSC, so we selected 4 most frequent mutation sites (404, 614, 1,662, 3,554) detected in multiple cancers, which may have implications for protein function, to be validated by Sanger sequencing (Figures 3E, F). This evidence concerns the gene FAT1 and cancer.