In the present study, we identified a potential complementary sequence of miR-4735-3p in SLC40A1 3′-UTR and determined that the miR-4735-3p mimic significantly reduced SLC40A1 expression, resulting in iron overload, lipid peroxidation, and ferroptosis in human ccRCC cell lines. The gene discussed is SLC40A1; the disease is nonpapillary renal cell carcinoma.