To decide the neighbor genes’ biological interaction network of these 20 stemness-related genes in HNSCC, we identified the top 10 frequent alterations were PIK3CA, FGF3, FGF19, FGF4, DVL3, P3H2, GNB4, COL22A1, COL14A1 and PLOD2. Phosphoinositide 3-kinase (PI3 K) and serine/threonine kinase AKT pathway regulate cellular functions such as proliferation, cell survival, and differentiation (Engelman et al., 2006). The gene discussed is FGF4; the disease is head and neck squamous cell carcinoma.