Constipation severity (Rome-IV criteria, p = 0.001; CSS, p = 0.022; BSS, p = 0.027), cognitive impairment (MoCA total score, p = 0.041; Mild Cognitive Impairment, p = 0.017), physical activity (IPAQ score, p = 0.017), pain (chronic pain, p < 0.001; pain severity, p < 0.001) and pharmacological therapies (Levodopa, p = 0.030; COMT inhibitor, p = 0.009) all showed a statistically significant association with changes in beta diversity. The gene discussed is COMT; the disease is constipation disorder.