EGFR and colon carcinoma: Many examples are given by the extensive research on liquid biopsies of various cancers [103]; for instance, Takur et al. reported the detection of specific colon carcinoma-associated mutations in plasma-derived EV-DNA; Lee et al. tested the sensitivity in detecting epidermal growth factor receptor (EGFR) mutations in EVs-DNA from BALF of patients with advanced non-small cell lung cancer (NSCLC) by targeted next-generation sequencing (NGS) of both tissue DNA and vesicular DNA [104].