The best TCER candidate identified in the CHO display system, CL-7467, exhibited highest binding affinity of 3.4 nM (KD), which is an about 400-fold increase over the parental TCR variant, and also showed highest anti-tumor cell reactivity together with the most pronounced release of perforin, granzyme B, IFNγ, and IL-2. Here, PRF1 is linked to neoplasm.