PRL and anxiety disorder: Recent human studies have shown disrupted functional connectivity of PFC‐related brain networks underlying anxiety disorders.[32, 33] The PrL‐BLA projection was examined as it is involved in environmental stress‐coping regulation.[2] Using a dual‐virus labeling system in which an AAV‐Retro‐Cre vector was transfected into the anterior BLA, followed by a second AAV‐expressing GCaMP6s flanked by a double inverted orientation (DIO) sequence into the PrL region, BLA‐projecting PrL neurons were labeled and recorded in vivo (Figure4a,b).