Despite the similar age between patients with AIS and MIA harboring ERBB2 20ins and those with ERBB2 SNVs (Fig. 4D), patients with AIS and MIA harboring ERBB2 20ins were significantly younger than those harboring other mutations, including BRAF kinase mutations and MAP2K1 indels (Fig. S10A, P < 0.001). The gene discussed is MAP2K1; the disease is androgen insensitivity syndrome.