The most prevalent mutation subtype of ERBB2 20ins from our cohort was p.A775_G776insYVMA (71.0%, 137/193), which had comparable detection rate across AIS (68.0%, 34/50), MIA (76.1%, 51/67), and IAC (68.4%, 52/76). Here, ERBB2 is linked to androgen insensitivity syndrome.