Along the differentiation trajectory, early DEG were associated with ECM-receptor interaction and TGFβ signaling as well as focal adhesion, while late in this trajectory expressed genes were associated with dilated cardiomyopathy, syndecan 4 pathway, translation and the BDNF signaling pathway (Fig. 5e). The gene discussed is TGFB1; the disease is dilated cardiomyopathy.