Out of the curiosity, we found that in breast cancer cells glutamine deprivation had no effect on the levels of NEDD8 and few neddylation enzymes, including E1 heterodimer NAE1/APPBP1, and UBA3/NAEβ, and two E2s, UBE2M and UBE2F (Supplementary Fig. 5h, i), whereas ectopic expression of NEDD8, UBE2M or UBE2F had no effect on the levels of SPOP or ASCT2 (Supplementary Fig. 5j, k). This evidence concerns the gene NEDD8 and breast cancer.