NAE1 and breast carcinoma: We also found that neither the SPOP-ASCT2 axis, nor NEDD8 and few neddylation enzymes, including E1 heterodimer NAE1/APPBP1, and UBA3/NAEβ, and two E2s, UBE2M and UBE2F is subjected to regulation by glucose deprivation in breast cancer cells (Supplementary Fig. 5b, c).