These results suggested that Th1-type CD4+ T-cells (CD4+ IFN-γ+) were predominantly at the early infection stage, whereas Th2-type CD4+ T-cells (CD4+ IL4+) were induced significantly higher at the middle/late stage, suggesting an inflammatory Th1 response gradually converting into a mixed Th1/Th2 response later. The gene discussed is IFNG; the disease is infection.