BCSC percentage in SUM159PT cultures was positively associated with FOXO1 expression with statistical significance when transfecting cells with FOXO1 over-expression plasmids or siRNAs (p=2.32E-3 for FOXO1-down, p=9.42E-3 for FOXO1-up, Fig. 3B,Supplementary Fig. 5C), which was consistent with the promotive role of FOXO1 on cancer stemness 34. The gene discussed is FOXO1; the disease is cancer.