Herein, the change of P62/SQSTM1 and LC3 II/I confirmed the inhibition of autophagy after the depletion of TBK1 in CRC cells (Figure 5C, Figure S6A-B), and the overexpression of TBK1 produced the opposite results (Figure 5C, Figure S6A-B). The gene discussed is MAP1LC3A; the disease is colorectal carcinoma.