Western blotting demonstrated that LY2109761 not only effectively reduced the phosphorylation of Smad2/3 but also prevented their nuclear translocation, indicating that the upregulation of the TGF-β signaling in tumor cells induced by M2-MDE coculture was successfully inhibited (Figures 5(a) and 5(b)). The gene discussed is SMAD2; the disease is neoplasm.