To track the S. aureus specific CD4+ T cell response mounted following a pulmonary infection, gDT-2 CD45.1+ T cells were transferred into C57BL/6 recipient mice (CD45.2+) prior to an intranasal infection with JKD6159-gD and the number of gDT-2 CD4+ T cells (CD4+Va3.2+CD45.1+) in both lymphoid and respiratory tissue was measured over time. Here, CD4 is linked to infection.