Finally, we assessed ferroptosis-related genes, including ATP5G3, RPL8, CS, and IREB2, as well as PTGS2 by qRT-PCR and found no changes in the expression of ATP5G3, RPL8, CS and IREB2 in retinas after IR injury, indicating that blood elements or longtime ischemia may be responsible for the upregulation of these genes in vivo [17]. Here, RPL8 is linked to ischemia.