Using imaging mass cytometry on 37 tumor cores representing different regions with a panel of 27 biomarkers, we extracted a single-cell database of 59,453 cells highlighting 4 cell lineages, marking stroma, lymphoid cells, myeloid cells, and HCC/hepatocytes, with different functional states, and marking expressions of PD-L1, Arginase-1 (Arg1), CCR6, Ki67, and CD163. The gene discussed is CD274; the disease is hepatocellular carcinoma.