FlowSOM algorithm further clustered these cells into 18 annotated phenotypes, including 1 stroma/architecture phenotype; 1 B cell phenotype; 1 CD4+ T cell phenotype; 1 CD8+ T cell phenotype; 1 double-positive cell phenotype; 1 regulatory T cell phenotype; 1 neutrophil (PD-L1+) cell phenotype; 5 myeloid/macrophage phenotypes; 5 HCC/hepatocyte phenotypes (Figures 2A, B); and 1 unannotatable/non-cell cluster (UA noncell). The gene discussed is CD4; the disease is hepatocellular carcinoma.