ACTA2 and neoplasm: Next, we employed lineage-specific markers to annotate clusters into major cell types of the tumor, including T cells (CD3G, CD4, CD8A, CD28, and IL7R), B cells (CD19 and CD20), myeloid cells (HLA-DRA and CD14), fibroblasts (THY1 and ACTA2), and tumor cells (EPCAM and S100A) (Figures S1A, B).