To validate expression of IL-12 and test whether tracer administration impacts downstream IL-12/IL-12R signaling, we performed qRT-PCR on flash frozen tumor tissue to measure expression of IL-12β and key proinflammatory cytokines indicative of active IL-12 signaling: IFNγ, TNFα, and IL-18 (Figure 5A). The gene discussed is IL12RB1; the disease is neoplasm.