The shedding of viral RNA into the culture supernatant was reduced by 94%, 99%, 83%, 78%, and 16% with the goat IFN-α incubated prior to CPIV3 infection for 24 h, 48 h, 5 d, 1 w, and 2 w, respectively (Figure 4C). This evidence concerns the gene IFNA1 and infection.