CD4 and progressive multifocal leukoencephalopathy: In both cases, cART was started early, within 7 days of HIV diagnosis (with tenofovir alafenamide, emtricitabine, darunavir/cobicistat plus dolutegravir in patient 4 and tenofovir alafenamide, emtricitabine, bictegravir plus darunavir/cobicistat in patient 5), but the patients showed severe immunosuppression at PML diagnosis (CD4 T cell count was 64/mmc in patient 4 and 15/mmc in patient 5).