ANXA5 and infection: Then, we performed further assays using Annexin-V/PI double-positive staining, and confirmed the apoptotic/necrosis process, which revealed the death signals triggered at D2 and D5 post-infection (as shown in Figure 2A–C), and that the apoptotic death signal (Figure 2B; primed at D2) can gradually become necrosis (Figure 2C; primed at D3).