IFNG and Increased total eosinophil count: rBCG::PTA vaccination also resulted in higher numbers of IFN-γ-secreting cells and lower numbers of IL-4-secreting cells in the lungs of allergic mice than pBCG vaccination, and the IL-12-IFN-γ axis was found to be key in the protective effect of rBCG::PTA, as investigated by the use of IL-12-deficient mice, in which the protective effects of the rBCG::PTA against eosinophilia and Th2 cytokine secretion in the lungs were partially reduced.