In the Mtb latently infected mouse model, the number of Ag85b/ESAT6-specific IFN-γ/IL-2 cells and the number of CFP10-specific IFN-γ cells decreased with increased immunization dose and time, while the number of CFP10-specific IL-2 cells increased in the infection-CT-AEC/BC02 group. Here, IFNG is linked to infection.