PRNP and variant Creutzfeldt-Jakob disease: When agents derived from cases of vCJD originating in five different countries (Canada, Italy, The Netherlands, UK, USA) were inoculated into a panel of selected well-characterized wild-type mice, the animals developed TSEs with similar histopathological lesion profiles, immunohistochemical changes, and PrPTSE banding patterns by Western blot (PrP “glycoforms”), supporting the hypothesis that a single strain of infectious agent (“classic” or C-BSE) caused all vCJD cases in North America and Europe and further suggesting that current clinical criteria for diagnosing vCJD are reliable [9].