The CuS-mediated NIR-II PTT under 1064 nm laser irradiation (0.6 W/cm2, 5 min) produced hyperthermia to significantly kill local tumor cells, and the plasmid triggered the high expression of IL-12 to promote the maturation of DCs, and the division and infiltration of CD8+ T cells for the repression of metastatic B16F10 tumors. This evidence concerns the gene CD8A and neoplasm.