In addition, Coumestrol effectively inhibited the cholinergic enzymes of AChE and BChE, which are target cholinergic enzymes for improving AD symptoms, with Ki values of 21.43 ± 3.70 and 21.65 ± 2.23 nM, respectively (Table 3, Figure 5A,B). Here, BCHE is linked to Alzheimer disease.