Given that NF-κB activation has been linked to increased HPSE expression [38], and our results show that RSV reduced NF-κB transcriptional activity in our experimental model, we analyzed the changes in heparanase activity in gastric cancer cells following RSV treatment following the degradation of a biotinylated-HS substrate (see Section 4). This evidence concerns the gene NFKB1 and gastric cancer.