In this work, we used the MTT assay to evaluate the cytotoxic effect of Apl-1 on different cancer cells, including leukemia (Molt 4 and K562) and prostate cancer (PC-3 and Du145) cells, as well as on normal human skin cells (CCD966SK) and normal rat macrophage cells (NR8383). The gene discussed is ADI1; the disease is prostate cancer.