SERPINE1 and cholangiocarcinoma: Furthermore, considerable amounts of serpin E1 and Macrophage migration inhibitory factor (MIF) could be determined in supernatants of mono-cultured HuCCT-1 and SC as well as in co-cultures of both CCA lines and SC (Figure 4B), while in supernatants of mono-cultured TFK-1 cells, no serpin E1 could be detected.