To investigate the effects of NAMPT gene knockdown as a potential target for the treatment of IDH mutant glioma, IDH1wt and IDH1R132H cells were transfected using two different esiRNAs targeting different sequences of NAMPT mRNA (esiNAMPT-A and esiNAMPT-B) to confirm the esiRNA specificity. The gene discussed is IDH1; the disease is central nervous system cancer.