The Transwell invasion assay (Figure 2C) and wound healing assay (Figure 2D) gave comparable results between 4T1 and a variety of human BC cell lines according to their histological and molecular subtypes, as well as their invasive potential: MDA-MB-231 (BasalB, triple-negative adenocarcinoma without amplified EGFR, highly invasive), T47D (Luminal, ER- and PR-positive invasive ductal carcinoma without HER2 amplification, with middle–low invasive potential) and MCF-7 (Luminal, ER- and PR-positive invasive ductal carcinoma without HER2 amplification, with low invasive potential). This evidence concerns the gene ERBB2 and breast cancer.