In the current research, we isolated the EVs produced by Colo-320 (colorectal adenocarcinoma) and SKOV-3 (ovarian adenocarcinoma) human cell lines and employed them as a model system to study the involvement of the adhesion molecule ALCAM/CD166 in EV interactions with—and in their subsequent cellular uptake by—cancer cells and by PMCs. This evidence concerns the gene ALCAM and cancer.