To determine the involvement of ALCAM/CD166 in the interactions between cancer-derived EVs and tumor cells, EVs isolated from parental SKOV-3 cells (“SKOV-3 EVs”, expressing abundant ALCAM) or from ALCAM-KO/SKOV-3 cells (“ALCAM-KO/SKOV-3 EVs”, lacking ALCAM expression) were first immobilized on plastic wells. Here, ALCAM is linked to cancer.