To test whether opto-activation of β2-AR in mPFC excitatory neurons can induce anxiety-like behavior, cre dependent AAV-ef1a-Dio-opto-β2-AR-EYFP or control virus together with AAV-CaMKIIα-cre were bilaterally injected into the mPFC and optical fibers were implanted (Figure 1d). The gene discussed is CAMK2A; the disease is Anxiety.