A comparison of 214 normal tissues with the average population risk for renal cancers and 157 (ANKRD34B) or 145 (ZIC1) adN tissue samples (i.e., cases with high cancer risk) revealed not only a clear age-dependent methylation pattern in normal and adN tissue, but also demonstrated methylation levels beyond the 99% prediction level of the linear regression, as defined by the analysis of low-risk normal tissues (Figure 3A,B: N and adN tissues). Here, CFD is linked to cancer.