NFKB1 and myocardial infarction: Mechanistically, multiple biochemical assays including Western blot, immunofluorescence, and imaging flow cytometry analysis showed that MI- and TNF-α-induced NF-κB p-p65 nuclear translocation was prevented by calcitriol, indicating that calcitriol exerts its anti-inflammatory effects by dampening NF-κB signaling in the context of MI.