In another study conducted in ESCC dealing with 28 cases of ESCC clinical samples, using an immunohistochemistry approach, Luz CC and colleagues [22] appraised the expression of HSP27 and HSP 70 at the same time, discouragingly observing that neither HSP27 nor HSP70 was markedly associated with gender, age, surgical margin, lymph node metastasis or tumor differentiation. This evidence concerns the gene HSPB1 and metastatic malignant neoplasm in the lymph nodes.