Consistent with these two articles, a statistically indistinguishable change in the proliferation rate of HNSCC cells was observed before and after HSP27 was silenced, as assayed by MTS with lentivirus-based short hairpin RNA (shRNA) plasmid, in a recent study by Zhu ZK and colleagues [34] in HNSCC. This evidence concerns the gene HSPB1 and head and neck squamous cell carcinoma.