To better understand the programs used by CD8+ memory T cells to infiltrate the MS brain, we assessed how RUNX3, EOMES and T-bet (co)expression is related to cytotoxic, tissue-homing and brain residency-associated features using blood, CSF and brain tissues from different MS cohorts, including treatment-naive patients genotyped for MS risk SNP rs6672420 (RUNX3). This evidence concerns the gene RUNX3 and myeloid sarcoma.